Genetic Analysis of the Individual of Chan do Lindeiro: Characterization of her Mitogenome and Situation of the Sample in the European Paleogenic Context

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Published: Mar 1, 2017

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Gloria González Fortes
Department of Biology, The University of York, YO10 5DD, Reino Unido. Institute for Biochemistry and Biology. University of Potsdam, 14476 Potsdam OT Golm, Alemania. Instituto Universitario de Xeoloxía. Universidade da Coruña. ESCI, Campus de Elviña s/n. 15071 A Coruña, España
United Kingdom
https://orcid.org/0000-0002-3700-7674
Aurora Grandal-d'Anglade
Instituto Universitario de Xeoloxía. Universidade da Coruña. ESCI, Campus de Elviña s/n. 15071 A Coruña, España
Spain
https://orcid.org/0000-0002-2087-4034
Juan Ramón Vidal Romaní
Instituto Universitario de Xeoloxía. Universidade da Coruña. ESCI, Campus de Elviña s/n. 15071 A Coruña
Spain
https://orcid.org/0000-0001-7158-4945
Michael Hofreiter
Department of Biology, The University of York, YO10 5DD, Reino Unido. Institute for Biochemistry and Biology. University of Potsdam, 14476 Potsdam OT Golm, Alemania
Germany
https://orcid.org/0000-0003-0441-4705
Vol. 39 (2017), Articles, pages 111-127
DOI: https://doi.org/10.17979/cadlaxe.2017.39.0.3558
Submitted: Jul 5, 2018 Published: Mar 1, 2017
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Abstract

In this work the first results of a paleogenetic study are presented in the bone remains of Elba, the mesolithic woman of Chan do Lindeiro (Pedrafita do Cebreiro, Lugo). The study was performed at the level of complete mitochondrial DNA and of nuclear markers related to phenotypic traits, such as pigmentation and lactose tolerance. The method is based on enrichment by hybridization capture of mitochondrial and genomic DNA, followed by high throughput sequencing. The coverage obtained for the genomic markers was insufficient, but the mitochondrial genome was recovered by 99%. The Chan do Lindeiro woman belongs to the haplogroup U, characteristic of the European hunter gatherers and within this, in the subhaplotype U5b1, whose origin is located in the Iberian Peninsula some 16-20000 years ago.

Keywords:

ancient mitochondrial DNA, NGS sequencing, Mesolithic, Chan do Lindeiro, Galicia ADN mitocondrioal antiguo, secuencias NGS, Mesolítico, Chan do Lindeiro, Galicia

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